Modificación secuencial del bagazo de cebada mediante ultrasonido e hidrólisis enzimática

Autores/as

DOI:

https://doi.org/10.19136/jobs.a12n33.6677

Palabras clave:

Hidrólisis enzimática, Ultrasonido, Bagazo de cebada, Propiedades funcionales

Resumen

El bagazo de malta de cebada (BSG), subproducto abundante de la industria cervecera, contiene fibra dietética y compuestos fenólicos con potencial funcional. Este estudio evaluó la modificación del BSG mediante un tratamiento secuencial consistente en ultrasonido (US, 80% amplitud, 30 min) seguido de hidrólisis enzimática con Driselasa por 1 y 4 h (HE, 1-4h) para mejorar sus propiedades funcionales y antioxidantes. La densidad aparente permaneció estable entre tratamientos (0.286-0.313 g/cm³). El US incrementó la capacidad de hinchamiento (15%), retención de agua (22%) y retención de aceite (54%) comparado con el BSG nativo. Los tratamientos secuenciales US→HE atenuaron parcialmente estos efectos. El contenido de fenoles totales disminuyó en muestras tratadas (31-45%), mientras que la capacidad antirradical DPPH aumentó en US→HE (14%) y la actividad ABTS apareció únicamente en muestras tratadas, maximizándose con US→HE-4h. Los tratamientos liberan compuestos fenólicos más reactivos pese a la reducción del contenido total. La modificación del BSG proporciona ingredientes mejorados para el desarrollo de alimentos funcionales.

Referencias

[1] Granato, D., Barba, F. J., Bursać Kovačević, D., Lorenzo, J. M., Cruz, A. G., & Putnik, P. (2020). Functional foods: Product development, technological trends, efficacy testing, and safety. Annual Review of Food Science and Technology, 11, 93-118. https://doi.org/10.1146/annurev-food-032519-051708 DOI: https://doi.org/10.1146/annurev-food-032519-051708

[2] Shahidi, F., & Ambigaipalan, P. (2018). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of Functional Foods, 18, 820-897. https://doi.org/10.1016/j.jff.2015.06.018 DOI: https://doi.org/10.1016/j.jff.2015.06.018

[3] Galanakis, C. M. (2012). Recovery of high added-value components from food wastes: Conventional, emerging technologies and commercialized applications. Trends in Food Science & Technology, 26(2), 68-87. https://doi.org/10.1016/j.tifs.2012.03.003 DOI: https://doi.org/10.1016/j.tifs.2012.03.003

[4] Trevizan, J., Bido, G., Ferrari, A., & Felipe, D. (2021). Nutritional Composition of Malted Barley Residue from Brewery. The Journal of Men's Studies, 11, 27. https://doi.org/10.5539/jms.v11n1p27. DOI: https://doi.org/10.5539/jms.v11n1p27

[5] Ikram, S., Huang, L., Zhang, H., Wang, J., & Yin, M. (2017). Composition and nutrient value proposition of brewers spent grain. Journal of Food Science, 82(10), 2232-2242. https://doi.org/10.1111/1750-3841.13794. DOI: https://doi.org/10.1111/1750-3841.13794

[6] Verni, M., Pontonio, E., Krona, A., Jacob, S., Pinto, D., Rinaldi, F., Verardo, V., Díaz-De-Cerio, E., Coda, R., & Rizzello, C. (2020). Bioprocessing of Brewers’ Spent Grain Enhances Its Antioxidant Activity: Characterization of Phenolic Compounds and Bioactive Peptides. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.01831. DOI: https://doi.org/10.3389/fmicb.2020.01831

[7] Bonifácio-Lopes T, Vilas Boas AA, Coscueta ER, Costa EM, Silva S, Campos D, Teixeira JA, Pintado M. Bioactive extracts from brewer's spent grain. Food Funct. 2020 Oct 21;11(10):8963-8977. doi: 10.1039/d0fo01426e. Erratum in: Food Funct. 2021 Nov 29;12(23):12162. doi: 10.1039/d1fo90072b. PMID: 33001088. DOI: https://doi.org/10.1039/D0FO01426E

[8] Lynch, K., Strain, C., Johnson, C., Patangia, D., Stanton, C., Koç, F., Gil-Martínez, J., O'riordan, P., Sahin, A., Ross, R., & Arendt, E. (2021). Extraction and characterisation of arabinoxylan from brewers spent grain and investigation of microbiome modulation potential. European Journal of Nutrition, 60, 4393 - 4411. https://doi.org/10.1007/s00394-021-02570-8. DOI: https://doi.org/10.1007/s00394-021-02570-8

[9] Forssell, P., Kontkanen, H., Schols, H., Hinz, S., Eijsink, V., Treimo, J., Robertson, J., Waldron, K., Faulds, C., & Buchert, J. (2008). Hydrolysis of Brewers' Spent Grain by Carbohydrate Degrading Enzymes. Journal of The Institute of Brewing, 114, 306-314. https://doi.org/10.1002/J.2050-0416.2008.TB00774.X. DOI: https://doi.org/10.1002/j.2050-0416.2008.tb00774.x

[10] Alonso-Riaño, P., Diez, M., Blanco, B., Beltrán, S., Trigueros, E., & Benito-Román, Ó. (2020). Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration. Antioxidants, 9. https://doi.org/10.3390/antiox9030265. DOI: https://doi.org/10.3390/antiox9030265

[11] Martín‐García, B., Pasini, F., Verardo, V., Díaz-De-Cerio, E., Tylewicz, U., Gómez-Caravaca, A., & Caboni, M. (2019). Optimization of Sonotrode Ultrasonic-Assisted Extraction of Proanthocyanidins from Brewers’ Spent Grains. Antioxidants, 8. https://doi.org/10.3390/antiox8080282. DOI: https://doi.org/10.3390/antiox8080282

[12] Qian, J., Chen, D., Zhang, Y., Gao, X., Xu, L., Guan, G., & Wang, F. (2023). Ultrasound-Assisted Enzymatic Protein Hydrolysis in Food Processing: Mechanism and Parameters. Foods, 12. https://doi.org/10.3390/foods12214027. DOI: https://doi.org/10.3390/foods12214027

[13] Naibaho, J., Korzeniowska, M., Wojdyło, A., Figiel, A., Yang, B., Laaksonen, O., Foste, M., Vilu, R., & Viiard, E. (2021). Fiber modification of brewers' spent grain by autoclave treatment to improve its properties as a functional food ingredient. LWT, 149, 111877. https://doi.org/10.1016/j.lwt.2021.111877 DOI: https://doi.org/10.1016/j.lwt.2021.111877

[14] Pramana, Y. S., Yulianto, A., Paramitasari, D., Supriyanti, A., & Elisa, S. (2023). Comparison of Dietary Fiber Characteristics Prepared from Cassava Pulp Using Acid-Heat Treatment and Extrusion Process. 1246. https://doi.org/10.1088/1755-1315/1246/1/012047 DOI: https://doi.org/10.1088/1755-1315/1246/1/012047

[15] Yamazaki, E., Murakami, K., & Kurita, O. (2005). Easy preparation of dietary fiber with the high water-holding capacity from food sources. Plant Foods for Human Nutrition, 60(1), 17–23. https://doi.org/10.1007/S11130-005-2537-9 DOI: https://doi.org/10.1007/s11130-005-2537-9

[16] Chumark, P., Khunawat, P., Sanvarinda, Y., Phornchirasilp, S., Morales, N. P., Phivthongngam, L., Ratanachamnong, P., Srisawat, S. and Pongrapeeporn, K. S. 2008. The in vitro and ex vivo antioxidant properties, hypolipidaemic and antioatheroscherotic activities of water extract of Moringa oleifera Lam Leaves. Journal of Ethnopharmacology. 116:439-446 DOI: https://doi.org/10.1016/j.jep.2007.12.010

[17] Siddhuraju, P. y Becker, K. 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agro-climatic origins of drumstick tree (Moringa oleífera Lam.). J. Agric. Food. Chem. 51(8) 2144-2155. DOI: https://doi.org/10.1021/jf020444+

[18] Naibaho, J., & Korzeniowska, M. (2021). The variability of physico-chemical properties of brewery spent grain from 8 different breweries. Heliyon, 7. https://doi.org/10.1016/j.heliyon.2021.e06583. DOI: https://doi.org/10.1016/j.heliyon.2021.e06583

[19] Devos, C., Bampouli, A., Brozzi, E., Stefanidis, G., Dusselier, M., Van Gerven, T., & Kuhn, S. (2024). Ultrasound mechanisms and their effect on solid synthesis and processing: a review. Chemical Society Reviews, 54, 85 - 115. https://doi.org/10.1039/d4cs00148f. DOI: https://doi.org/10.1039/D4CS00148F

[20] Zhang, T., Tang, H., Vavylonis, D., & Cosgrove, D. (2019). Disentangling loosening from softening: insights into primary cell wall structure. The Plant journal: for cell and molecular biology. https://doi.org/10.1111/tpj.14519. DOI: https://doi.org/10.1111/tpj.14519

[21] Deng, L., Chen, Q., Ohm, J., Islam, S., Rao, J., Jin, Z., & Xu, M. (2024). Upcycling soybean meal through enzymatic conversion of insoluble fiber into soluble dietary fiber enhanced by ball milling.. Journal of food science. https://doi.org/10.1111/1750-3841.17185. DOI: https://doi.org/10.1111/1750-3841.17185

[22] Düsenberg, B., Schmidt, J., Sensoy, I., & Bück, A. (2023). Flowability of plant based food powders: Almond, chestnut, chickpea, coconut, hazelnut and rice. Journal of Food Engineering. https://doi.org/10.1016/j.jfoodeng.2023.111606. DOI: https://doi.org/10.1016/j.jfoodeng.2023.111606

[23] Oladunmoye, O., Akinoso, R., & Olapade, A. (2010). EVALUATION OF SOME PHYSICAL–CHEMICAL PROPERTIES OF WHEAT, CASSAVA, MAIZE AND COWPEA FLOURS FOR BREAD MAKING. Journal of Food Quality, 33, 693-708. https://doi.org/10.1111/J.1745-4557.2010.00351.X. DOI: https://doi.org/10.1111/j.1745-4557.2010.00351.x

[24] Zhang, G., Cai, D., Wang, M., Zhang, C., Zhang, J., & Wu, Z. (2013). Microstructural modification of diatomite by acid treatment, high-speed shear, and ultrasound. Microporous and Mesoporous Materials, 165, 106-112. https://doi.org/10.1016/J.MICROMESO.2012.08.005. DOI: https://doi.org/10.1016/j.micromeso.2012.08.005

[25] Huang, Y., Li, C., Zheng, S., Fu, X., Huang, Q., Liu, G., & Chen, Q. (2024). Influence of Three Modification Methods on the Structure, Physicochemical, and Functional Properties of Insoluble Dietary Fiber from Rosa roxburghii Tratt Pomace. Molecules, 29. https://doi.org/10.3390/molecules29092111. DOI: https://doi.org/10.3390/molecules29092111

[26] Sun, X., Liu, S., Zhang, X., Tao, Y., Boczkaj, G., Yoon, J., & Xuan, X. (2021). Recent advances in hydrodynamic cavitation-based pretreatments of lignocellulosic biomass for valorization.. Bioresource technology, 126251. https://doi.org/10.1016/j.biortech.2021.126251. DOI: https://doi.org/10.1016/j.biortech.2021.126251

[27] Li, S., Hu, N., Zhu, J., Zheng, M., Liu, H., & Liu, J. (2022). Influence of modification methods on physicochemical and structural properties of soluble dietary fiber from corn bran. Food Chemistry: X, 14. https://doi.org/10.1016/j.fochx.2022.100298. DOI: https://doi.org/10.1016/j.fochx.2022.100298

[28] Dendegh, T., Ukeyima, M., & Dendegh, R. (2019). Proximate Composition, Functional and Sensory Properties of Pearl Millet, Soy Flour and Baobab Fruit Pulp Composite Flour as a Complementary Food. Asian Food Science Journal. https://doi.org/10.9734/AFSJ/2019/V8I329992. DOI: https://doi.org/10.9734/afsj/2019/v8i329992

[29] Jeong, M., & Cho, S. (2023). Effect of pH-shifting on the water holding capacity and gelation properties of mung bean protein isolate. Food research international, 177, 113912. https://doi.org/10.1016/j.foodres.2023.113912. DOI: https://doi.org/10.1016/j.foodres.2023.113912

[30] Madison, M., Coward-Kelly, G., Liang, C., Karim, M., Falls, M., & Holtzapple, M. (2017). Mechanical pretreatment of biomass – Part I: Acoustic and hydrodynamic cavitation. Biomass & Bioenergy, 98, 135-141. https://doi.org/10.1016/J.BIOMBIOE.2017.01.007. DOI: https://doi.org/10.1016/j.biombioe.2017.01.007

[31] Zhu, N., Liu, Y., Zhang, X., Gao, H., Zeng, J., Yang, J., Song, J., Li, X., & Zhao, T. (2024). Effect of enzymatic hydrolysis of arabinoxylan on the quality of frozen dough during the subfreezing process. Journal of the science of food and agriculture. https://doi.org/10.1002/jsfa.13433. DOI: https://doi.org/10.1002/jsfa.13433

[32] Wang, Z., Wang, L., Yu, X., Wang, X., Zheng, Y., Hu, X., Zhang, P., Sun, Q., Wang, Q., & Li, N. (2023). Effect of polysaccharide addition on food physical properties: A review.. Food chemistry, 431, 137099. https://doi.org/10.1016/j.foodchem.2023.137099. DOI: https://doi.org/10.1016/j.foodchem.2023.137099

[33] Hassan, Z., Imran, M., Ahmad, M., & Khan, M. (2021). Ultrasound-Assisted Modification of Insoluble Dietary Fiber from Chia (Salvia hispanica L.) Seeds. Journal of Food Quality. https://doi.org/10.1155/2021/5035299. DOI: https://doi.org/10.1155/2021/5035299

[34] Sang, J., Li, L., Wen, J., Gu, Q., Wu, J., Yu, Y., Xu, Y., Fu, M., & Lin, X. (2021). Evaluation of the Structural, Physicochemical and Functional Properties of Dietary Fiber Extracted from Newhall Navel Orange By-Products. Foods, 10. https://doi.org/10.3390/foods10112772. DOI: https://doi.org/10.3390/foods10112772

[35] Zhang, M., Liao, A., Thakur, K., Huang, J., Zhang, J., & Wei, Z. (2019). Modification of wheat bran insoluble dietary fiber with carboxymethylation, complex enzymatic hydrolysis and ultrafine comminution. Food chemistry, 297, 124983. https://doi.org/10.1016/j.foodchem.2019.124983. DOI: https://doi.org/10.1016/j.foodchem.2019.124983

[36] Türker, İ., & Isleroglu, H. (2023). Dietary fibers of fenugreek seeds: Storage stability and food application. Food and Health. https://doi.org/10.3153/fh23015. DOI: https://doi.org/10.3153/FH23015

[37] Zhu, R., Tan, S., Wang, Y., Zhang, L., & Huang, L. (2023). Physicochemical Properties and Hypolipidemic Activity of Dietary Fiber from Rice Bran Meal Obtained by Three Oil-Production Methods. Foods, 12. https://doi.org/10.3390/foods12193695. DOI: https://doi.org/10.3390/foods12193695

[38] Iadecola, R., Ciccoritti, R., Ceccantoni, B., Bellincontro, A., & Amoriello, T. (2022). Optimization of Phenolic Compound Extraction from Brewers’ Spent Grain Using Ultrasound Technologies Coupled with Response Surface Methodology. Sustainability. https://doi.org/10.3390/su14063309. DOI: https://doi.org/10.3390/su14063309

[39] Carciochi, R., Sologubik, C., Fernández, M., Manrique, G., & D'Alessandro, L. (2018). Extraction of Antioxidant Phenolic Compounds from Brewer’s Spent Grain: Optimization and Kinetics Modeling. Antioxidants, 7. https://doi.org/10.3390/antiox7040045. DOI: https://doi.org/10.3390/antiox7040045

[40] Kitrytė, V., Šaduikis, A., & Venskutonis, P. (2015). Assessment of antioxidant capacity of brewer’s spent grain and its supercritical carbon dioxide extract as sources of valuable dietary ingredients. Journal of Food Engineering, 167, 18-24. https://doi.org/10.1016/J.JFOODENG.2014.12.005. DOI: https://doi.org/10.1016/j.jfoodeng.2014.12.005

[41] Zhong, X., Zhang, S., Wang, H., Yang, J., Li, L., Zhu, J., & Liu, Y. (2022). Ultrasound-alkaline combined extraction improves the release of bound polyphenols from pitahaya (Hylocereus undatus 'Foo-Lon') peel: Composition, antioxidant activities and enzyme inhibitory activity. Ultrasonics Sonochemistry, 90. https://doi.org/10.1016/j.ultsonch.2022.106213. DOI: https://doi.org/10.1016/j.ultsonch.2022.106213

[42] Kornpointner, C., Scheibelreiter, J., & Halbwirth, H. (2022). Snailase: A Promising Tool for the Enzymatic Hydrolysis of Flavonoid Glycosides From Plant Extracts. Frontiers in Plant Science, 13. https://doi.org/10.3389/fpls.2022.889184. DOI: https://doi.org/10.3389/fpls.2022.889184

[43] Vilas-Franquesa, A., Fryganas, C., Casertano, M., Montemurro, M., & Fogliano, V. (2023). Upcycling mango peels into a functional ingredient by combining fermentation and enzymatic-assisted extraction. Food chemistry, 434, 137515. https://doi.org/10.1016/j.foodchem.2023.137515. DOI: https://doi.org/10.1016/j.foodchem.2023.137515

[44] Flavia Rafaela Ludka, Ana Beatriz Klosowski, Giovani Almeida Camargo, Aline Silva Justo, Evelyn Assis Andrade, Flávio Luis Beltrame, Juliana Bonametti Olivato, Brewers' spent grain extract as antioxidants in starch-based active biopolymers, International Journal of Food Science and Technology, Volume 59, Issue 1, January 2024, Pages 142–150, https://doi.org/10.1111/ijfs.16785 DOI: https://doi.org/10.1111/ijfs.16785

Descargas

Publicado

2026-04-30

Número

Sección

Artículo científico

Cómo citar

Primo Mora, Ángel R., Aparicio Saguilán, A., Páramo Calderón, D. E., Sánchez Ocampo, P. M., & Utrilla Coello, R. G. (2026). Modificación secuencial del bagazo de cebada mediante ultrasonido e hidrólisis enzimática. JOURNAL OF BASIC SCIENCES, 12(33), 27-41. https://doi.org/10.19136/jobs.a12n33.6677